Recombinant ptxA

Alternative Names:Pertussis toxin subunit 1 Protein, PTX S1 Protein, Islet-activating protein S1 Protein, IAP S1 Protein, NAD-dependent ADP-ribosyltransferase Protein
Catalogue No.:P8909Uniprot ID:P04977
Host:E.ColiExpression Region:35-269
Mol. Weight:26.2 KDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant ptxA
Catalogue No.
P8909
Species
Bordetella pertussis
Host
E.Coli
Expression Region
35-269
Mol. Weight
26.2 KDa
Tags
N-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Pertussis toxin subunit 1 Protein, PTX S1 Protein, Islet-activating protein S1 Protein, IAP S1 Protein, NAD-dependent ADP-ribosyltransferase Protein
SDS-PAGE